Your Kinase Connection
 

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 Your Kinase Connection
 
 
 
11 New Cell-Based No Wash
Kits for Monitoring Protein

Phosphorylation
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AlphaScreen® SureFire® a highly sensitive, simple to use no-wash technology that can be used to:

  • Measure endogenous proteins in recombinant & endogenous cells- including primary cells
  • Grow your cells and detect all-in-one well
  • Measure multiple targets from one cell lysate
  PerkinElmer Tools for Kinase Screening
Detection Instruments
Automated Liquid Handling


Microplates


Service

PerkinElmer's AlphaScreen SureFire platform is a no wash “mix and read” technology that eliminates the cumbersome multiple wash steps typically needed in many assay protocols, as well as in Western Blot analysis.

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The no wash capability makes these assays amenable to automation for high-throughput screening. Moreover, AlphaScreen SureFire has the advantage that it measures full-length, endogeonous protein phosphorylation in a cell based format, offering high sensitivity and precision, even in complex samples.

The newest assay kits include:

  1. Phospho-ALK (Tyr1586)
  2. Phospho-ALK (Tyr1604)
  3. Phospho-Chk-1 (Ser345)
  4. Phospho-c-Jun (Ser63)
  5. Phospho-c-Jun (Ser73)
  6. Phospho-EGF Receptor (Tyr1068)
  7. Phospho-Elk-1 (Ser383)
  8. Phospho-ErbB2 (Tyr1221/1222)
  9. Phospho-IGF-1 Receptor (Tyr1135/1136)
  10. Phospho-Insulin Receptor (Tyr1150/1151)
  11. Phospho-mTOR (Ser2448)

The rapidly growing portfolio of AlphaScreen SureFire cell-based assays include 39 kits covering the following applications:

  • MAPK Signaling
  • AKT Signaling
  • Translational Control
  • NF-κβ Signaling
  • JAK/STAT Signaling
  • TGFβ/SMAD Signaling
  • Receptor Tyrosine Kinase (RTK) Signaling

Please visit www.perkinelmer.com/surefire to view the full product listing.

Validated on EnVision With Alpha
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PerkinElmer assays are validated on the EnVision® Multilabel Plate Reader with Alpha, for verification of performance and quality. EnVision Multilabel Plate Readers are fast, sensitive and versatile benchtop readers that deliver optimized performance in every application and for every label.

©2008 PerkinElmer, Inc. All rights reserved. AlphaScreen and EnVision are registered marks of PerkinElmer, Inc.